Rapid immunopurification of mitochondria for metabolite profiling and absolute quantification of matrix metabolites
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Author(s)
Freinkman, Elizaveta
Chen, Walter W.
Sabatini, David
Date Issued
September 2017
Journal
Nature Protocols
Publisher
Springer Nature
Citation
Chen, Walter W, Elizaveta Freinkman, and David M Sabatini. “Rapid Immunopurification of Mitochondria for Metabolite Profiling and Absolute Quantification of Matrix Metabolites.” Nature Protocols 12, no. 10 (September 29, 2017): 2215–2231.
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Author's final manuscript
Abstract
Mitochondria carry out numerous metabolic reactions that are critical to cellular homeostasis. Here we present a protocol for interrogating mitochondrial metabolites and measuring their matrix concentrations. Our workflow uses high-affinity magnetic immunocapture to rapidly purify HA-tagged mitochondria from homogenized mammalian cells in ∼12 min. These mitochondria are extracted with methanol and water. Liquid chromatography and mass spectrometry (LC/MS) is used to determine the identities and mole quantities of mitochondrial metabolites using authentic metabolite standards and isotopically labeled internal standards, whereas the corresponding mitochondrial matrix volume is determined via immunoblotting, confocal microscopy of intact cells, and volumetric analysis. Once all values have been obtained, the matrix volume is combined with the aforementioned mole quantities to calculate the matrix concentrations of mitochondrial metabolites. With shortened isolation times and improved mitochondrial purity when compared with alternative methods, this LC/MS-compatible workflow allows for robust profiling of mitochondrial metabolites and serves as a strategy generalizable to the study of other mammalian organelles. Once all the necessary reagents have been prepared, quantifying the matrix concentrations of mitochondrial metabolites can be accomplished within a week.
MIT Department
Institute for Medical Engineering and Science
Massachusetts Institute of Technology. Department of Biology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/NPROT.2017.104
