Hot embossing for fabrication of a microfluidic 3D cell culture
Name
Hot embossing for fabrication of a microfluidic 3D cell culture platform - jeon 2010 biomed.pdf
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434.88 KB
Format
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Checksum (MD5)
870ae6e3ca1dd99f4ccd35b7b1226244
Author(s)
Jeon, Jessie S.
Chung, Seok
Kamm, Roger Dale
Charest, Joseph L.
Date Issued
November 2010
Journal
Biomedical Microdevices
Publisher
Springer Netherlands
Citation
Jeon, Jessie S. et al. “Hot embossing for fabrication of a microfluidic 3D cell culture platform.” Biomedical Microdevices 13.2 (2010): 325-333.
Version
Author's final manuscript
Abstract
Clinically relevant studies of cell function in vitro require a physiologically-representative microenvironment possessing aspects such as a 3D extracellular matrix (ECM) and controlled biochemical and biophysical parameters. A polydimethylsiloxane (PDMS) microfluidic system with a 3D collagen gel has previously served for analysis of factors inducing different responses of cells in a 3D microenvironment under controlled biochemical and biophysical parameters. In the present study, applying the known commercially-viable manufacturing methods to a cyclic olefin copolymer (COC) material resulted in a microfluidic device with enhanced 3D gel capabilities, controlled surface properties, and improved potential to serve high-volume applications. Hot embossing and roller lamination molded and sealed the microfluidic device. A combination of oxygen plasma and thermal treatments enhanced the sealing, ensured proper placement of the 3D gel, and created controlled and stable surface properties within the device. Culture of cells in the new device indicated no adverse effects of the COC material or processing as compared to previous PDMS devices. The results demonstrate a methodology to transition microfludic devices for 3D cell culture from scientific research to high-volume applications with broad clinical impact.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1007/s10544-010-9496-0
