Biocompatibility and biofilm inhibition of N,N-hexyl,methyl-polyethylenimine bonded to Boston Keratoprosthesis materials
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Klibanov_Biocompatibility and biofilm.pdf
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Checksum (MD5)
97112e6057393768e2b87f56954a1005
Author(s)
Behlau, Irmgard
Mukherjee, Koushik
Todani, Amit
Tisdale, Ann S.
Cade, Fabiano
Wang, Liqiang
Leonard, Elizabeth M.
Zakka, Fouad R.
Gilmore, Michael S.
Jakobiec, Frederick A.
Date Issued
September 2011
Journal
Biomaterials
Publisher
Elsevier
Citation
Behlau, Irmgard, Koushik Mukherjee, Amit Todani, Ann S. Tisdale, Fabiano Cade, Liqiang Wang, Elizabeth M. Leonard, et al. “Biocompatibility and Biofilm Inhibition of N,N-Hexyl,methyl-Polyethylenimine Bonded to Boston Keratoprosthesis Materials.” Biomaterials 32, no. 34 (December 2011): 8783–8796.
Version
Author's final manuscript
Abstract
The biocompatibility and antibacterial properties of N,N-hexyl,methyl-polyethylenimine (HMPEI) covalently attached to the Boston Keratoprosthesis (B-KPro) materials was evaluated. By means of confocal and electron microscopies, we observed that HMPEI-derivatized materials exert an inhibitory effect on biofilm formation by Staphylococcus aureus clinical isolates, as compared to the parent poly(methyl methacrylate) (PMMA) and titanium. There was no additional corneal epithelial cell cytotoxicity of HMPEI-coated PMMA compared to that of control PMMA in tissue cultures in vitro. Likewise, no toxicity or adverse reactivity was detected with HMPEI-derivatized PMMA or titanium compared to those of the control materials after intrastromal or anterior chamber implantation in rabbits in vivo.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemistry
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Creative Commons Attribution-Noncommercial-NoDerivatives
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DOI of Published Version
https://doi.org/10.1016/j.biomaterials.2011.08.010
