Biomimetic Culture Reactor for Whole-Lung Engineering
Name
Raredon-2016-Biomimetic Culture Reactor for Wh.pdf
Size
903.35 KB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Raredon, Micha Sam Brickman
Rocco, Kevin A.
Gheorghe, Ciprian P.
Sivarapatna, Amogh
Ghaedi, Mahboobe
Balestrini, Jenna L.
Raredon, Thomas L.
Calle, Elizabeth A.
Niklason, Laura E.
Date Issued
April 2016
Journal
BioResearch Open Access
Publisher
Mary Ann Lieber
Citation
Raredon, Micha Sam Brickman et al. “Biomimetic Culture Reactor for Whole-Lung Engineering.” BioResearch Open Access 5.1 (2016): 72–83.
Version
Final published version
Abstract
Decellularized organs are now established as promising scaffolds for whole-organ regeneration. For this work to reach therapeutic practice, techniques and apparatus are necessary for doing human-scale clinically applicable organ cultures. We have designed and constructed a bioreactor system capable of accommodating whole human or porcine lungs, and we describe in this study relevant technical details, means of assembly and operation, and validation. The reactor has an artificial diaphragm that mimics the conditions found in the chest cavity in vivo, driving hydraulically regulated negative pressure ventilation and custom-built pulsatile perfusion apparatus capable of driving pressure-regulated or volume-regulated vascular flow. Both forms of mechanical actuation can be tuned to match specific physiologic profiles. The organ is sealed in an elastic artificial pleura that mounts to a support architecture. This pleura reduces the fluid volume required for organ culture, maintains the organ's position during mechanical conditioning, and creates a sterile barrier allowing disassembly and maintenance outside of a biosafety cabinet. The combination of fluid suspension, negative-pressure ventilation, and physiologic perfusion allows the described system to provide a biomimetic mechanical environment not found in existing technologies and especially suited to whole-organ regeneration. In this study, we explain the design and operation of this apparatus and present data validating intended functions.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Terms of Use
Creative Commons Attribution 4.0 International License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1089/biores.2016.0006
