Advances in Engineering and Application of Optogenetic Indicators for Neuroscience
Name
applsci-09-00562-v2.pdf
Size
2.5 MB
Format
Adobe PDF
Checksum (MD5)
b416a8b208f628234288264207b44e8d
Author(s)
Subach, Fedor V.
Piatkevich, Kiryl D.
Murdock, Mitchell H.
Subach, Fedor V.
Murdock, Mitchell
Date Issued
February 2019
Journal
Applied Sciences
Publisher
Multidisciplinary Digital Publishing Institute (MDPI)
Citation
Piatkevich, Kiryl D. et al. "Advances in Engineering and Application of Optogenetic Indicators for Neuroscience." Applied Sciences 9, 3 (February 2019): 562 © 2016 The Authors
Version
Final published version
Abstract
Our ability to investigate the brain is limited by available technologies that can record biological processes in vivo with suitable spatiotemporal resolution. Advances in optogenetics now enable optical recording and perturbation of central physiological processes within the intact brains of model organisms. By monitoring key signaling molecules noninvasively, we can better appreciate how information is processed and integrated within intact circuits. In this review, we describe recent efforts engineering genetically-encoded fluorescence indicators to monitor neuronal activity. We summarize recent advances of sensors for calcium, potassium, voltage, and select neurotransmitters, focusing on their molecular design, properties, and current limitations. We also highlight impressive applications of these sensors in neuroscience research. We adopt the view that advances in sensor engineering will yield enduring insights on systems neuroscience. Neuroscientists are eager to adopt suitable tools for imaging neural activity in vivo, making this a golden age for engineering optogenetic indicators. Keywords: optogenetic tools; neuroscience; calcium sensor; voltage sensor; neurotransmitters
MIT Department
Massachusetts Institute of Technology. Media Laboratory
Terms of Use
Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.3390/app9030562
