Stimuli-responsive microwells for formation and retrieval of cell aggregates
Name
nihms-272430.pdf
Description
Accepted version
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868.56 KB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Tekin, Halil
Anaya, Michael
Brigham, Mark D
Nauman, Claire
Langer, Robert
Khademhosseini, Ali
Date Issued
2010
Journal
Lab on a Chip
Publisher
Royal Society of Chemistry (RSC)
Citation
Tekin, H., et al. "Stimuli-Responsive Microwells for Formation and Retrieval of Cell Aggregates." Lab on a Chip 10 18 (2010): 2411-18.
Version
Author's final manuscript
Abstract
Generating cell aggregates is beneficial for various applications ranging from biotechnology to regenerative therapies. Previously, poly(ethylene glycol) (PEG) microwells have been demonstrated as a potentially useful method for generating controlled-size cell aggregates. In addition to controlling cell aggregate size and homogeneity, the ability to confine cell aggregates on glass adhesive substrates and subsequently retrieve aggregates from microwells for further experimentation and analysis could be beneficial for various applications. However, it is often difficult to retrieve cell aggregates from these microwells without the use of digestive enzymes. This study describes the stable formation of cell aggregates in responsive microwells with adhesive substrates and their further retrieval in a temperature dependent manner by exploiting the stimuli responsiveness of these microwells. The responsive polymer structure of the arrays can be used to thermally regulate the microwell diameters causing a mechanical force on the aggregates, subsequently facilitating the retrieval of cell aggregates from the microwells with high efficiency compared to PEG arrays. This approach can be potentially integrated into high-throughput systems and may become a versatile tool for various applications that require aggregate formation and experimentation, such as tissue engineering, drug discovery, and stem cell biology. © 2010 The Royal Society of Chemistry.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Department of Chemical Engineering
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Biology
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1039/c004732e
