M13 Virus‐Based Framework for High Fluorescence Enhancement
Name
M13 Virus-based Framework for High Fluorescence Enhancement.pdf
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1.29 MB
Format
Adobe PDF
Checksum (MD5)
17db016027a0de50ea9dba79184a7fbf
Author(s)
Qi, Jifa
deQuilettes, Dane W.
Huang, Mantao
Lin, Ching-Wei
Bardhan, Neelkanth Manoj
Dang, Xiangnan
Bulović, Vladimir
Belcher, Angela M
Huang, Shengnan,Ph.D.Massachusetts Institute of Technology.
Date Issued
May 2019
Journal
Small
Publisher
Wiley
Citation
Huang, Shengnan et al. "M13 Virus‐Based Framework for High Fluorescence Enhancement." Small 15, 28 (May 2019): 1901233 © 2019 WILEY‐VCH Verlag GmbH & Co
Version
Author's final manuscript
Abstract
Fluorescence imaging is a powerful tool for studying biologically relevant macromolecules, but its applicability is often limited by the fluorescent probe, which must demonstrate both high site‐specificity and emission efficiency. In this regard, M13 virus, a versatile biological scaffold, has previously been used to both assemble fluorophores on its viral capsid with molecular precision and to also target a variety of cells. Although M13‐fluorophore systems are highly selective, these complexes typically suffer from poor molecular detection limits due to low absorption cross‐sections and moderate quantum yields. To overcome these challenges, a coassembly of the M13 virus, cyanine 3 dye, and silver nanoparticles is developed to create a fluorescent tag capable of binding with molecular precision with high emissivity. Enhanced emission of cyanine 3 of up to 24‐fold is achieved by varying nanoparticle size and particle‐fluorophore separation. In addition, it is found that the fluorescence enhancement increases with increasing dye surface density on the viral capsid. Finally, this highly fluorescent probe is applied for in vitro staining of E. coli . These results demonstrate an inexpensive framework for achieving tuned fluorescence enhancements. The methodology developed in this work is potentially amendable to fluorescent detection of a wide range of M13/cell combinations.
MIT Department
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Massachusetts Institute of Technology. Department of Biological Engineering
Koch Institute for Integrative Cancer Research at MIT
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1002/smll.201901233
