Combinatorial Development of Biomaterials for Clonal Growth of Human Pluripotent Stem Cells
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Jaenisch_Combinatorial development.pdf
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2.01 MB
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Author(s)
Mei, Ying
Saha, Krishanu
Bogatyrev, Said R.
Yang, Jing
Hook, Andrew L.
Kalcioglu, Zeynep Ilke
Cho, Seung Woo
Mitalipova, Maisam
Pyzocha, Neena
Rojas, Fredrick P.
Date Issued
August 2010
Journal
Nature Materials
Publisher
Nature Publishing Group
Citation
Mei, Ying et al. “Combinatorial Development of Biomaterials for Clonal Growth of Human Pluripotent Stem Cells.” Nature Materials 9.9 (2010): 768–778.
Version
Author's final manuscript
Abstract
Both human embryonic stem cells and induced pluripotent stem cells can self-renew indefinitely in culture; however, present methods to clonally grow them are inefficient and poorly defined for genetic manipulation and therapeutic purposes. Here we develop the first chemically defined, xeno-free, feeder-free synthetic substrates to support robust self-renewal of fully dissociated human embryonic stem and induced pluripotent stem cells. Material properties including wettability, surface topography, surface chemistry and indentation elastic modulus of all polymeric substrates were quantified using high-throughput methods to develop structure–function relationships between material properties and biological performance. These analyses show that optimal human embryonic stem cell substrates are generated from monomers with high acrylate content, have a moderate wettability and employ integrin α[subscript v]β[subscript 3] and α[subscript v]β[subscript 5] engagement with adsorbed vitronectin to promote colony formation. The structure–function methodology employed herein provides a general framework for the combinatorial development of synthetic substrates for stem cell culture.
Description
July 3, 2012
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Koch Institute for Integrative Cancer Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/nmat2812
