Using buoyant mass to measure the growth of single cells
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Grossman_Using buoyant.pdf
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Author(s)
Godin, Michel
Son, Sungmin
Tzur, Amit
Jorgensen, Paul
Kirschner, Marc W.
Delgado, Francisco Feijo
Grover, William H.
Bryan, Andrea Kristine
Payer, Kristofor Robert
Grossman, Alan D.
Date Issued
April 2010
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
Godin, Michel, Francisco Feijó Delgado, Sungmin Son, William H Grover, Andrea K Bryan, Amit Tzur, Paul Jorgensen, et al. “Using buoyant mass to measure the growth of single cells.” Nature Methods 7, no. 5 (April 11, 2010): 387-390.
Version
Author's final manuscript
Abstract
We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the 'instantaneous' growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in a fraction of a complete cell cycle. We found that for individual cells of Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae and mouse lymphoblasts, heavier cells grew faster than lighter cells.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Microsystems Technology Laboratories
Koch Institute for Integrative Cancer Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/nmeth.1452
