Cell Patterning with Mucin Biopolymers
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Stocker_Cell patterning.pdf
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Author(s)
Crouzier, T.
Jang, H.
Ahn, J.
Stocker, Roman
Ribbeck, Katharina
Date Issued
September 2013
Journal
Biomacromolecules
Publisher
American Chemical Society (ACS)
Citation
Crouzier, T., H. Jang, J. Ahn, R. Stocker, and K. Ribbeck. “Cell Patterning with Mucin Biopolymers.” Biomacromolecules 14, no. 9 (September 9, 2013): 3010–3016.
Version
Author's final manuscript
Abstract
The precise spatial control of cell adhesion to surfaces is an endeavor that has enabled discoveries in cell biology and new possibilities in tissue engineering. The generation of cell-repellent surfaces currently requires advanced chemistry techniques and could be simplified. Here we show that mucins, glycoproteins of high structural and chemical complexity, spontaneously adsorb on hydrophobic substrates to form coatings that prevent the surface adhesion of mammalian epithelial cells, fibroblasts, and myoblasts. These mucin coatings can be patterned with micrometer precision using a microfluidic device, and are stable enough to support myoblast differentiation over seven days. Moreover, our data indicate that the cell-repellent effect is dependent on mucin-associated glycans because their removal results in a loss of effective cell-repulsion. Last, we show that a critical surface density of mucins, which is required to achieve cell-repulsion, is efficiently obtained on hydrophobic surfaces, but not on hydrophilic glass surfaces. However, this limitation can be overcome by coating glass with hydrophobic fluorosilane. We conclude that mucin biopolymers are attractive candidates to control cell adhesion on surfaces.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Civil and Environmental Engineering
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1021/bm400447z
