Static mechanical strain induces capillary endothelial cell cycle re-entry and sprouting
Name
nihms812339.pdf
Description
Accepted version
Size
1.32 MB
Format
Adobe PDF
Checksum (MD5)
f02d715171db0605a113128b9c86acf7
Author(s)
Zeiger, AS
Liu, FD
Durham, JT
Jagielska, A
Mahmoodian, R
Van Vliet, KJ
Herman, IM
Date Issued
2016
Journal
Physical Biology
Publisher
IOP Publishing
Citation
Zeiger, A. S., et al. "Static Mechanical Strain Induces Capillary Endothelial Cell Cycle Re-Entry and Sprouting." Phys Biol 13 4 (2016): 046006.
Version
Author's final manuscript
Abstract
© 2016 IOP Publishing Ltd. Vascular endothelial cells are known to respond to a range of biochemical and time-varying mechanical cues that can promote blood vessel sprouting termed angiogenesis. It is less understood how these cells respond to sustained (i.e., static) mechanical cues such as the deformation generated by other contractile vascular cells, cues which can change with age and disease state. Here we demonstrate that static tensile strain of 10%, consistent with that exerted by contractile microvascular pericytes, can directly and rapidly induce cell cycle re-entry in growth-arrested microvascular endothelial cell monolayers. S-phase entry in response to this strain correlates with absence of nuclear p27, a cyclin-dependent kinase inhibitor. Furthermore, this modest strain promotes sprouting of endothelial cells, suggesting a novel mechanical 'angiogenic switch'. These findings suggest that static tensile strain can directly stimulate pathological angiogenesis, implying that pericyte absence or death is not necessarily required of endothelial cell re-activation.
MIT Department
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1088/1478-3975/13/4/046006
