Sub-population analysis based on temporal features of high content images
Author(s)
Veronika, Merlin
Evans, James
Matsudaira, Paul
Rajapakse, Jagath
Welsch, Roy E
Date Issued
December 2009
Journal
BMC Bioinformatics
Publisher
BMC Bioinformatics
Citation
Veronika, Merlin, James Evans, Paul Matsudaira, Roy Welsch, and Jagath Rajapakse. “Sub-Population Analysis Based on Temporal Features of High Content Images.” BMC Bioinformatics 10, no. S15 (December 2009). © 2009 Veronika et al.
Version
Final published version
Abstract
Background: High content screening techniques are increasingly used to understand the regulation and progression of cell motility. The demand of new platforms, coupled with availability of terabytes of data has challenged the traditional technique of identifying cell populations by manual methods and resulted in development of high-dimensional analytical methods. Results: In this paper, we present sub-populations analysis of cells at the tissue level by using dynamic features of the cells. We used active contour without edges for segmentation of cells, which preserves the cell morphology, and autoregressive modeling to model cell trajectories. The sub-populations were obtained by clustering static, dynamic and a combination of both features. We were able to identify three unique sub-populations in combined clustering. Conclusion: We report a novel method to identify sub-populations using kinetic features and demonstrate that these features improve sub-population analysis at the tissue level. These advances will facilitate the application of high content screening data analysis to new and complex biological problems.
MIT Department
Sloan School of Management
Terms of Use
Creative Commons Attribution 2.0 Generic license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1186/1471-2105-10-S15-S4
