Cell type–specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function
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Feng_Cell type-specific.pdf
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Author(s)
Zhao, Shengli
Ting, Jonathan Thomas
Atallah, Hicham
Qiu, Li
Tan, Jie
Gloss, Bernd
Augustine, George J.
Luo, Minmin
Deisseroth, Karl
Graybiel, Ann M.
Date Issued
August 2011
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
Zhao, Shengli, Jonathan T Ting, Hisham E Atallah, Li Qiu, Jie Tan, Bernd Gloss, George J Augustine, et al. “Cell Type–specific Channelrhodopsin-2 Transgenic Mice for Optogenetic Dissection of Neural Circuitry Function.” Nature Methods 8, no. 9 (August 7, 2011): 745–752.
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Author's final manuscript
Abstract
Optogenetic methods have emerged as powerful tools for dissecting neural circuit connectivity, function and dysfunction. We used a bacterial artificial chromosome (BAC) transgenic strategy to express the H134R variant of channelrhodopsin-2, ChR2(H134R), under the control of cell type–specific promoter elements. We performed an extensive functional characterization of the newly established VGAT-ChR2(H134R)-EYFP, ChAT-ChR2(H134R)-EYFP, Tph2-ChR2(H134R)-EYFP and Pvalb(H134R)-ChR2-EYFP BAC transgenic mouse lines and demonstrate the utility of these lines for precisely controlling action-potential firing of GABAergic, cholinergic, serotonergic and parvalbumin-expressing neuron subsets using blue light. This resource of cell type–specific ChR2(H134R) mouse lines will facilitate the precise mapping of neuronal connectivity and the dissection of the neural basis of behavior.
Description
Available in PMC 2011 October 12.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
McGovern Institute for Brain Research at MIT
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DOI of Published Version
https://doi.org/10.1038/nmeth.1668
