Isolation and characterization of bacterial polyhydroxybutyrate inclusions
Name
71301456-MIT.pdf
Description
Full printable version
Size
21.21 MB
Format
Adobe PDF
Checksum (MD5)
19597345e1f396de8572e81d65fe54b7
Author(s)
Kshetry, Nina
Advisor(s)
Cho Kyun Rha.
Date Issued
2006
Publisher
Massachusetts Institute of Technology
Abstract
Polyhydroxybutyrate (PHB) is a carbon reserve found in some bacteria, and under nutrient limiting conditions accumulates intracellularly in the form of inclusion bodies. These inclusions contain proteins, and the PHB within the inclusions exists in an amorphous state. In this study a procedure to recover native PHB inclusions was developed, and the isolated inclusions were characterized using 13C NMR, western blotting, SDS-PAGE, atomic force microscopy, and fluorescence microscopy. A model for the structure of native PHB inclusions is proposed.
Description
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Civil and Environmental Engineering, 2006.
Includes bibliographical references (leaves 79-83).
Subjects
Civil and Environmental Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Civil and Environmental Engineering
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