In vivo and in vitro tracking of erosion in biodegradable materials using non-invasive fluorescence imaging
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Edelman_In vivo.pdf
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1.43 MB
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Author(s)
Artzi, Natalie
Oliva, Nuria
Puron, Cristina
Shitreet, Sagi
bon Ramos, Adriana
Artzi, Shay
Groothuis, Adam R.
Sahagian, Gary
Edelman, Elazer R
Date Issued
August 2011
Journal
Nature Materials
Publisher
Nature Publishing Group
Citation
Artzi, Natalie et al. “In Vivo and in Vitro Tracking of Erosion in Biodegradable Materials Using Non-invasive Fluorescence Imaging.” Nature Materials 10.9 (2011): 704–709.
Version
Author's final manuscript
Abstract
The design of erodible biomaterials relies on the ability to program the in vivo retention time, which necessitates real-time monitoring of erosion. However, in vivo performance cannot always be predicted by traditional determination of in vitro erosion[superscript 1, 2] , and standard methods sacrifice samples or animals[superscript 3], preventing sequential measures of the same specimen. We harnessed non-invasive fluorescence imaging to sequentially follow in vivo material-mass loss to model the degradation of materials hydrolytically (PEG:dextran hydrogel) and enzymatically (collagen). Hydrogel erosion rates in vivo and in vitro correlated, enabling the prediction of in vivo erosion of new material formulations from in vitro data. Collagen in vivo erosion was used to infer physiologic in vitro conditions that mimic erosive in vivo environments. This approach enables rapid in vitro screening of materials, and can be extended to simultaneously determine drug release and material erosion from a drug-eluting scaffold, or cell viability and material fate in tissue-engineering formulations.
Description
Author Manuscript 2012 March 1.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Harvard University--MIT Division of Health Sciences and Technology
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/nmat3095
