An intrinsically disordered yeast prion arrests the cell cycle by sequestering a spindle pole body component
Name
Treusch-2012-An intrinsically dis.pdf
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2.86 MB
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Author(s)
Treusch, Sebastian
Lindquist, Susan
Date Issued
April 2012
Journal
Journal of Cell Biology
Publisher
Rockefeller University Press, The
Citation
Treusch, S., and S. Lindquist. “An Intrinsically Disordered Yeast Prion Arrests the Cell Cycle by Sequestering a Spindle Pole Body Component.” The Journal of Cell Biology 197.3 (2012): 369–379. Copyright © 2012 by The Rockefeller University Press
Version
Final published version
Abstract
Intrinsically disordered proteins play causative roles in many human diseases. Their overexpression is toxic in many organisms, but the causes of toxicity are opaque. In this paper, we exploit yeast technologies to determine the root of toxicity for one such protein, the yeast prion Rnq1. This protein is profoundly toxic when overexpressed but only in cells carrying the endogenous Rnq1 protein in its [RNQ[superscript +]] prion (amyloid) conformation. Surprisingly, toxicity was not caused by general proteotoxic stress. Rather, it involved a highly specific mitotic arrest mediated by the Mad2 cell cycle checkpoint. Monopolar spindles accumulated as a result of defective duplication of the yeast centrosome (spindle pole body [SPB]). This arose from selective Rnq1-mediated sequestration of the core SPB component Spc42 in the insoluble protein deposit (IPOD). Rnq1 does not normally participate in spindle pole dynamics, but it does assemble at the IPOD when aggregated. Our work illustrates how the promiscuous interactions of an intrinsically disordered protein can produce highly specific cellular toxicities through illicit, yet highly specific, interactions with the proteome.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported
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DOI of Published Version
https://doi.org/10.1083/jcb.201108146
