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Design of genetic circuits that are robust to resource competition
Name
COISB_2021-1.pdf
Description
Accepted version
Size
1.22 MB
Format
Adobe PDF
Checksum (MD5)
56adffeb57d6a90103882fd5e1bc22bc
Author(s)
McBride, Cameron D
Grunberg, Theodore W
Del Vecchio, Domitilla
Date Issued
2021
Journal
Current Opinion in Systems Biology
Publisher
Elsevier BV
Citation
McBride, Cameron D, Grunberg, Theodore W and Del Vecchio, Domitilla. 2021. "Design of genetic circuits that are robust to resource competition." Current Opinion in Systems Biology, 28.
Version
Author's final manuscript
Abstract
The ability to engineer genetic circuits in living cells has tremendous potential in many applications, from health, to energy, to bio-manufacturing. Although substantial efforts have gone into design approaches that make circuits robust to variable cellular context, context dependence of genetic circuits remains a significant hurdle. We review intra-cellular resource competition, one culprit of context dependence, and summarize recent efforts toward design approaches to mitigate it. We classify these approaches into two main groups: global control and local control. In the former, the pool of resources is regulated to meet the demand, and in the latter, individual modules are regulated to be robust to variability in the pool of resources. Within each group, we highlight both feedback and feedforward implementations.
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
10.1016/J.COISB.2021.100357
