T Cell Receptor Internalization from the Immunological Synapse Is Mediated by TC21 and RhoG GTPase-Dependent Phagocytosis
Name
Martínez-Martín-2011-T Cell Receptor Inte.pdf
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2.36 MB
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Author(s)
Cemerski, Saso
Delgado, Pilar
Turner, Martin
Heuser, John
Irvine, Darrell J.
Huang, Bonnie
Shaw, Andrey
Martinez-Martin, Nuria
Fernandez-Arenas, Elena
Alarcon, Balbino
Date Issued
August 2011
Journal
Immunity
Publisher
Elsevier
Citation
Martinez-Martin, Nuria, Elena Fernandez-Arenas, Saso Cemerski, Pilar Delgado, Martin Turner, John Heuser, Darrell J. Irvine, et al. “T Cell Receptor Internalization from the Immunological Synapse Is Mediated by TC21 and RhoG GTPase-Dependent Phagocytosis.” Immunity 35, no. 2 (August 2011): 208–222. © 2011 Elsevier Inc.
Version
Final published version
Abstract
The immunological synapse (IS) serves a dual role for sustained T cell receptor (TCR) signaling and for TCR downregulation. TC21 (Rras2) is a RRas subfamily GTPase that constitutively associates with the TCR and is implicated in tonic TCR signaling by activating phosphatidylinositol 3-kinase. In this study, we demonstrate that TC21 both cotranslocates with the TCR to the IS and is necessary for TCR internalization from the IS through a mechanism dependent on RhoG, a small GTPase previously associated with phagocytosis. Indeed, we found that the TCR triggers T cells to phagocytose 1–6 μm beads through a TC21- and RhoG-dependent pathway. We further show that TC21 and RhoG are necessary for the TCR-promoted uptake of major histocompatibility complex (MHC) from antigen-presenting cells. Therefore, TC21 and RhoG dependence underlie the existence of a common phagocytic mechanism that drives TCR internalization from the IS together with its peptide-MHC ligand.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
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DOI of Published Version
https://doi.org/10.1016/j.immuni.2011.06.003
