Palm Fruit Bioactives modulate human astrocyte activity in vitro altering the cytokine secretome reducing levels of TNFα, RANTES and IP-10
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Author(s)
Weinberg, Robert P
Koledova, Vera V
Schneider, Kirsten K
Sambandan, T G
Grayson, Adlai R
Zeidman, Gal
Artamonova, Anastasia
Sambanthamurthi, Ravigadevi
Fairus, Syed
Sinskey, Anthony J
Date Issued
November 2018
Journal
Scientific Reports
Publisher
Springer Science and Business Media LLC
Citation
Weinberg, Robert P. et al. “Palm Fruit Bioactives Modulate Human Astrocyte Activity in Vitro Altering the Cytokine Secretome Reducing Levels of TNFα, RANTES and IP-10.” Scientific Reports 8, 1 (November 2018): 16423 © 2018 The Author(s)
Version
Final published version
Abstract
Neurodegenerative diseases, including Alzheimer’s disease and Parkinson’s disease, are becoming more prevalent and an increasing burden on society. Neurodegenerative diseases often arise in the milieu of neuro-inflammation of the brain. Reactive astrocytes are key regulators in the development of neuro-inflammation. This study describes the effects of Palm Fruit Bioactives (PFB) on the behavior of human astrocytes which have been activated by IL-1β. When activated, the astrocytes proliferate, release numerous cytokines/chemokines including TNFα, RANTES (CCL5), IP-10 (CXCL10), generate reactive oxygen species (ROS), and express specific cell surface biomarkers such as the Intercellular Adhesion Molecule (ICAM), Vascular Cellular Adhesion Molecule (VCAM) and the Neuronal Cellular Adhesion Molecule (NCAM). Interleukin 1-beta (IL-1β) causes activation of human astrocytes with marked upregulation of pro-inflammatory genes. We show significant inhibition of these pro-inflammatory processes when IL-1β-activated astrocytes are exposed to PFB. PFB causes a dose-dependent and time-dependent reduction in specific cytokines: TNFα, RANTES, and IP-10. We also show that PFB significantly reduces ROS production by IL-1β-activated astrocytes. Furthermore, PFB also reduces the expression of ICAM and VCAM, both in activated and naïve human astrocytes in vitro. Since reactive astrocytes play an essential role in the neuroinflammatory state preceding neurodegenerative diseases, this study suggests that PFB may have a potential role in their prevention and/or treatment.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Biomaterials Science and Engineering Laboratory
Massachusetts Institute of Technology. Department of Biological Engineering
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DOI of Published Version
https://doi.org/10.1038/s41598-018-34763-3
