Investigations into the role of protein phosphatases in the EGFR signaling network
Name
313410867-MIT.pdf
Description
Full printable version
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3.23 MB
Format
Adobe PDF
Checksum (MD5)
6001dee030d0126036849bc5c3601249
Author(s)
Lu, Kuojung Gordon
Advisor(s)
Peter K. Sorger.
Date Issued
2008
Publisher
Massachusetts Institute of Technology
Abstract
Protein phosphatases regulate the phosphorylation state of intracellular signaling molecules in conjunction with protein kinases. In order to better understand the role of phosphatases in the ErbB signaling network, experimental studies were carried out to validate assays and phosphatase inhibitors for gathering dynamic, system-wide data of phosphatase activity. We verified the use of In-Cell Westerns and phospho-ErbB ELISAs for these studies, as well as the use of different cell lines and both general and specific phosphatase inhibitors in system-wide experiments. The phosphatase inhibitors we used perturbed cellular systems in complex ways that can help elucidate the regulation and activity of specific phosphatases in the ErbB signaling pathway in the future.
Description
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Biology, 2008.
Includes bibliographical references (leaves 39-43).
Subjects
Biology.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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