A Septin-Dependent Diffusion Barrier at Dendritic Spine Necks
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Ewers-2014-A Septin-Dependent D.pdf
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Author(s)
Ewers, Helge
Tada, Tomoko
Petersen, Jennifer D.
Racz, Bence
Sheng, Morgan
Choquet, Daniel
Date Issued
December 2014
Journal
PLoS ONE
Publisher
Public Library of Science
Citation
Ewers, Helge, Tomoko Tada, Jennifer D. Petersen, Bence Racz, Morgan Sheng, and Daniel Choquet. “A Septin-Dependent Diffusion Barrier at Dendritic Spine Necks.” Edited by Lin Mei. PLoS ONE 9, no. 12 (December 10, 2014): e113916.
Version
Final published version
Abstract
Excitatory glutamatergic synapses at dendritic spines exchange and modulate their receptor content via lateral membrane diffusion. Several studies have shown that the thin spine neck impedes the access of membrane and solute molecules to the spine head. However, it is unclear whether the spine neck geometry alone restricts access to dendritic spines or if a physical barrier to the diffusion of molecules exists. Here, we investigated whether a complex of septin cytoskeletal GTPases localized at the base of the spine neck regulates diffusion across the spine neck. We found that, during development, a marker of the septin complex, Septin7 (Sept7), becomes localized to the spine neck where it forms a stable structure underneath the plasma membrane. We show that diffusion of receptors and bulk membrane, but not cytoplasmic proteins, is slower in spines bearing Sept7 at their neck. Finally, when Sept7 expression was suppressed by RNA interference, membrane molecules explored larger membrane areas. Our findings indicate that Sept7 regulates membrane protein access to spines.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Picower Institute for Learning and Memory
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0113916
