Lectin-Seq: A method to profile lectin-microbe interactions in native communities
Name
sciadv.add8766.pdf
Description
Published version
Size
1.58 MB
Format
Adobe PDF
Checksum (MD5)
ac2aef0e2aa31f213628d210c3a82bbb
Author(s)
McPherson, Robert L
Isabella, Christine R
Walker, Rebecca L
Sergio, Dallis
Bae, Sunhee
Gaca, Tony
Raman, Smrithi
Nguyen, Le Thanh Tu
Wesener, Darryl A
Halim, Melanie
Date Issued
July 28, 2023
Journal
Science Advances
Publisher
American Association for the Advancement of Science
Citation
Robert L. McPherson et al. , Lectin-Seq: A method to profile lectin-microbe interactions in native communities. Sci. Adv.9, eadd8766 (2023).
Version
Final published version
Abstract
Soluble human lectins are critical components of innate immunity. Genetic models suggest that lectins influence host-resident microbiota, but their specificity for commensal and mutualist species is understudied. Elucidating lectins’ roles in regulating microbiota requires an understanding of which microbial species they bind within native communities. To profile human lectin recognition, we developed Lectin-Seq. We apply Lectin-Seq to human fecal microbiota using the soluble mannose-binding lectin (MBL) and intelectin-1 (hItln1). Although each lectin binds a substantial percentage of the samples (10 to 20%), the microbial interactomes of MBL and hItln1 differ markedly in composition and diversity. MBL binding is highly selective for a small subset of species commonly associated with humans. In contrast, hItln1’s interaction profile encompasses a broad range of lower-abundance species. Our data uncover stark differences in the commensal recognition properties of human lectins.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
Creative Commons Attribution-NonCommercial
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DOI of Published Version
https://doi.org/10.1126/sciadv.add8766
