Mir-290–295 deficiency in mice results in partially penetrant embryonic lethality and germ cell defects
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Medeiros-2011-Aug-Mir-290-295 deficien.pdf
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Author(s)
Medeiros, Lea Ann
Dennis, Lucas M.
Gill, Mark E.
Houbaviy, Hristo
Markoulaki, Styliani
Fu, Dongdong
White, Amy C.
Kirak, Oktay
Sharp, Phillip A.
Jaenisch, Rudolf
Date Issued
August 2011
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publisher
National Academy of Sciences (U.S.)
Citation
Medeiros, L. A. et al. “Mir-290-295 Deficiency in Mice Results in Partially Penetrant Embryonic Lethality and Germ Cell Defects.” Proceedings of the National Academy of Sciences 108.34 (2011): 14163–14168. Web. ©2011 by the National Academy of Sciences.
Version
Final published version
Abstract
Mir-290 through mir-295 (mir-290–295) is a mammalian-specific microRNA (miRNA) cluster that, in mice, is expressed specifically in early embryos and embryonic germ cells. Here, we show that mir-290–295 plays important roles in embryonic development as indicated by the partially penetrant lethality of mutant embryos. In addition, we show that in surviving mir-290–295-deficient embryos, female but not male fertility is compromised. This impairment in fertility arises from a defect in migrating primordial germ cells and occurs equally in male and female mutant animals. Male mir-290–295−/− mice, due to the extended proliferative lifespan of their germ cells, are able to recover from this initial germ cell loss and are fertile. Female mir-290–295−/− mice are unable to recover and are sterile, due to premature ovarian failure.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
Koch Institute for Integrative Cancer Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1073/pnas.1111241108
