RNA sequencing reveals two major classes of gene expression levels in metazoan cells
Author(s)
Hebenstreit, Daniel
Fang, Miaoqing
Charoensawan, Varodom
van Oudenaarden, Alexander
Teichmann, Sarah A.
Date Issued
June 2011
Journal
Molecular Systems Biology
Publisher
Nature Publishing Group
Citation
Hebenstreit, Daniel et al. “RNA sequencing reveals two major classes of gene expression levels in metazoan cells.” Molecular Systems Biology 7 (2011)
Version
Author's final manuscript
Abstract
The expression level of a gene is often used as a proxy for determining whether the
protein or RNA product is functional in a cell or tissue. Therefore, it is of fundamental
importance to understand the global distribution of gene expression levels, and to be
able to interpret it mechanistically and functionally. Here we use RNA sequencing of
mouse Th2 cells, coupled with a range of other techniques, to show that all genes can
be separated, based on their expression abundance, into two distinct groups: one
group comprising of lowly expressed and putatively non-functional mRNAs, and the
other of highly expressed mRNAs with active chromatin marks at their promoters.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Physics
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/msb.2011.28
