Targeted optogenetic stimulation and recording of neurons in vivo using cell-type-specific expression of Channelrhodopsin-2
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Tsai_Targeted optogenetic.pdf
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Author(s)
Knoblich, Ulf
Zhang, Feng
Deisseroth, Karl
Tsai, Li-Huei
Cardin, Jessica A.
Carlen, Marie
Meletis, Konstantinos
Moore, Christopher I.
Date Issued
January 2010
Journal
Nature Protocols
Publisher
Nature Publishing Group
Citation
Cardin, Jessica A, Marie Carlen, Konstantinos Meletis, Ulf Knoblich, Feng Zhang, Karl Deisseroth, Li-Huei Tsai, and Christopher I Moore. “Targeted Optogenetic Stimulation and Recording of Neurons in Vivo Using Cell-Type-Specific Expression of Channelrhodopsin-2.” Nat Protoc 5, no. 2 (January 21, 2010): 247–254.
Version
Author's final manuscript
Abstract
A major long-term goal of systems neuroscience is to identify the different roles of neural subtypes in brain circuit function. The ability to causally manipulate selective cell types is critical to meeting this goal. This protocol describes techniques for optically stimulating specific populations of excitatory neurons and inhibitory interneurons in vivo in combination with electrophysiology. Cell type selectivity is obtained using Cre-dependent expression of the light-activated channel Channelrhodopsin-2. We also describe approaches for minimizing optical interference with simultaneous extracellular and intracellular recording. These optogenetic techniques provide a spatially and temporally precise means of studying neural activity in the intact brain and allow a detailed examination of the effect of evoked activity on the surrounding local neural network. Injection of viral vectors requires 30–45 min, and in vivo electrophysiology with optogenetic stimulation requires 1–4 h.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
McGovern Institute for Brain Research at MIT
Picower Institute for Learning and Memory
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1038/nprot.2009.228
