Responsive Micromolds for Sequential Patterning of Hydrogel Microstructures
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Langer_Responsive micromolds.pdf
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Author(s)
Tekin, Halil
Sanchez, Jefferson G.
Jones, Brianna J.
Camci-Unal, Gulden
Nichol, Jason W.
Khademhosseini, Ali
Tsinman, Tonia
Langer, Robert S
Date Issued
July 2011
Journal
Journal of the American Chemical Society
Publisher
American Chemical Society
Citation
Tekin, Halil, Tonia Tsinman, Jefferson G. Sanchez, et al. 2011. Responsive Micromolds for Sequential Patterning of Hydrogel Microstructures. Journal of the American Chemical Society 133(33): 12944–12947.
Version
Author's final manuscript
Abstract
Microscale hydrogels have been shown to be beneficial for various applications such as tissue engineering and drug delivery. A key aspect in these applications is the spatial organization of biological entities or chemical compounds within hydrogel microstructures. For this purpose, sequentially patterned microgels can be used to spatially organize either living materials to mimic biological complexity or multiple chemicals to design functional microparticles for drug delivery. Photolithographic methods are the most common way to pattern microscale hydrogels but are limited to photocrosslinkable polymers. So far, conventional micromolding approaches use static molds to fabricate structures, limiting the resulting shapes that can be generated. Herein, we describe a dynamic micromolding technique to fabricate sequentially patterned hydrogel microstructures by exploiting the thermoresponsiveness of poly(N-isopropylacrylamide)-based micromolds. These responsive micromolds exhibited shape changes under temperature variations, facilitating the sequential molding of microgels at two different temperatures. We fabricated multicompartmental striped, cylindrical, and cubic microgels that encapsulated fluorescent polymer microspheres or different cell types. These responsive micromolds can be used to immobilize living materials or chemicals into sequentially patterned hydrogel microstructures which may potentially be useful for a range of applications at the interface of chemistry, materials science and engineering, and biology.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Department of Physics
Koch Institute for Integrative Cancer Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1021/ja204266a
