Experience-dependent regulation of CaMKII activity within single visual cortex synapses in vivo
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Mower-2011-Experience-dependent.pdf
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Author(s)
Mower, Amanda F.
Yu, Hongbo
Majewska, Ania K.
Okamoto, Ken-Ichi
Hayashi, Yasunori
Sur, Mriganka
Kwok, Show Ming
Date Issued
December 2011
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publisher
National Academy of Sciences of the United States of America
Citation
Mower, A. F. et al. “Experience-dependent Regulation of CaMKII Activity Within Single Visual Cortex Synapses in Vivo.” Proceedings of the National Academy of Sciences 108.52 (2011): 21241–21246. Copyright ©2011 by the National Academy of Sciences
Version
Final published version
Abstract
Unbalanced visual input during development induces persistent alterations in the function and structure of visual cortical neurons. The molecular mechanisms that drive activity-dependent changes await direct visualization of underlying signals at individual synapses in vivo. By using a genetically engineered Förster resonance energy transfer (FRET) probe for the detection of CaMKII activity, and two-photon imaging of single synapses within identified functional domains, we have revealed unexpected and differential mechanisms in specific subsets of synapses in vivo. Brief monocular deprivation leads to activation of CaMKII in most synapses of layer 2/3 pyramidal cells within deprived eye domains, despite reduced visual drive, but not in nondeprived eye domains. Synapses that are eliminated in deprived eye domains have low basal CaMKII activity, implying a protective role for activated CaMKII against synapse elimination.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Picower Institute for Learning and Memory
RIKEN-MIT Center for Neural Circuit Genetics
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1073/pnas.1108261109
