Single-shot 20-fold expansion microscopy
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s41592-024-02454-9.pdf
Description
Published version
Size
10.46 MB
Format
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Checksum (MD5)
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Author(s)
Wang, Shiwei
Shin, Tay Won
Yoder, Harley B
McMillan, Ryan B
Su, Hanquan
Liu, Yixi
Zhang, Chi
Leung, Kylie S
Yin, Peng
Kiessling, Laura L
Date Issued
October 11, 2024
Journal
Nature Methods
Publisher
Springer Science and Business Media LLC
Citation
Wang, S., Shin, T.W., Yoder, H.B. et al. Single-shot 20-fold expansion microscopy. Nat Methods 21, 2128–2134 (2024).
Version
Final published version
Abstract
Expansion microscopy (ExM) is in increasingly widespread use throughout biology because its isotropic physical magnification enables nanoimaging on conventional microscopes. To date, ExM methods either expand specimens to a limited range (~4–10× linearly) or achieve larger expansion factors through iterating the expansion process a second time (~15–20× linearly). Here, we present an ExM protocol that achieves ~20× expansion (yielding <20-nm resolution on a conventional microscope) in a single expansion step, achieving the performance of iterative expansion with the simplicity of a single-shot protocol. This protocol, which we call 20ExM, supports postexpansion staining for brain tissue, which can facilitate biomolecular labeling. 20ExM may find utility in many areas of biological investigation requiring high-resolution imaging.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1038/s41592-024-02454-9
