Probing metal nanoparticles and assemblies with analytical ultracentrifugation
Name
666483709-MIT.pdf
Description
Full printable version
Size
9.16 MB
Format
Adobe PDF
Checksum (MD5)
db3b9926505b89902dbc2dee91781f4d
Author(s)
Carney, Randy (Randy Patrick)
Advisor(s)
Francesco Stellacci.
Alternative Title
Synthesis, characterization, and fractionation of cell penetrating gold nanoparticles
Date Issued
2010
Publisher
Massachusetts Institute of Technology
Abstract
Analytical Ultracentrifugation (AUC) is a powerful tool to obtain statistically relevant size and shape measurements for macromolecular systems. Metal nanoparticles coated by a ligand shell of thiolated molecules provide diverse functionality, from targeted cellular delivery to the formation of complex assemblies. Here I show that AUC can be used to determine particle size distribution, ligand shell density, shape, and hydrodynamic radius. It can also be used to probe complex mixtures of nanoparticle assemblies, from 2D dimers and chains, to 3D trimers, tetramers, and higher order assemblies, from a consideration of their hydrodynamic shape factor and its relation to the sedimentation coefficient. With AUC, the ease of sample preparation, ligand shell information, and dramatic increase in sample size are improvements compared with electron microscopy, and the ability to probe multiple, discrete absorbing wavelengths and globally analyze with interference information offers a measured improvement compared with dynamic light scattering (DLS). This work describes multiple calibrations and considerations as well as theoretical contributions concerning the application of AUC to nanoparticle systems
Description
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2010.
Includes bibliographical references (p. 42-43).
Subjects
Materials Science and Engineering.
MIT Department
Massachusetts Institute of Technology. Department of Materials Science and Engineering
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