Engineered yeast tolerance enables efficient production from toxified lignocellulosic feedstocks
Name
sciadv.abf7613.pdf
Description
Published version
Size
293.57 KB
Format
Adobe PDF
Checksum (MD5)
6ff2efd5a4ccba8fb344268806dd8f20
Author(s)
Lam, Felix H
Turanlı-Yıldız, Burcu
Liu, Dany
Resch, Michael G
Fink, Gerald R
Stephanopoulos, Gregory
Date Issued
June 25, 2021
Journal
Science Advances
Publisher
American Association for the Advancement of Science
Citation
Felix H. Lam et al. ,Engineered yeast tolerance enables efficient production from toxified lignocellulosic feedstocks.Sci. Adv.7, eabf7613 (2021).
Version
Final published version
Abstract
Lignocellulosic biomass remains unharnessed for the production of renewable fuels and chemicals due to challenges in deconstruction and the toxicity its hydrolysates pose to fermentation microorganisms. Here, we show in Saccharomyces cerevisiae that engineered aldehyde reduction and elevated extracellular potassium and pH are sufficient to enable near-parity production between inhibitor-laden and inhibitor-free feedstocks. By specifically targeting the universal hydrolysate inhibitors, a single strain is enhanced to tolerate a broad diversity of highly toxified genuine feedstocks and consistently achieve industrial-scale titers (cellulosic ethanol of >100 grams per liter when toxified). Furthermore, a functionally orthogonal, lightweight design enables seamless transferability to existing metabolically engineered chassis strains: We endow full, multifeedstock tolerance on a xylose-consuming strain and one producing the biodegradable plastics precursor lactic acid. The demonstration of “drop-in” hydrolysate competence enables the potential of cost-effective, at-scale biomass utilization for cellulosic fuel and nonfuel products alike.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Whitehead Institute for Biomedical Research
Terms of Use
Creative Commons Attribution-Noncommercial
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DOI of Published Version
https://doi.org/10.1126/sciadv.abf7613
