Differentiated human stem cells resemble fetal, not adult, β cells
Name
Hrvatin-2014-Differentiated human.pdf
Size
941.1 KB
Format
Adobe PDF
Checksum (MD5)
0a434340c15656d0f1d60086160d4dca
Author(s)
Hrvatin, Sinisa
O'Donnell, Charles William
Deng, Francis
Millman, Jeffrey R.
Pagliuca, Felicia Walton
DiIorio, Philip
Rezania, Alireza
Gifford, David K.
Melton, Douglas A.
Date Issued
February 2014
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Hrvatin, S., C. W. O’Donnell, F. Deng, J. R. Millman, F. W. Pagliuca, P. DiIorio, A. Rezania, D. K. Gifford, and D. A. Melton. “Differentiated Human Stem Cells Resemble Fetal, Not Adult, β Cells.” Proceedings of the National Academy of Sciences 111, no. 8 (February 10, 2014): 3038–3043.
Version
Final published version
Abstract
Human pluripotent stem cells (hPSCs) have the potential to generate any human cell type, and one widely recognized goal is to make pancreatic β cells. To this end, comparisons between differentiated cell types produced in vitro and their in vivo counterparts are essential to validate hPSC-derived cells. Genome-wide transcriptional analysis of sorted insulin-expressing (INS[superscript +]) cells derived from three independent hPSC lines, human fetal pancreata, and adult human islets points to two major conclusions: (i) Different hPSC lines produce highly similar INS[superscript +] cells and (ii) hPSC-derived INS[superscript +] (hPSC-INS[superscript +]) cells more closely resemble human fetal β cells than adult β cells. This study provides a direct comparison of transcriptional programs between pure hPSC-INS[superscript +] cells and true β cells and provides a catalog of genes whose manipulation may convert hPSC-INS[superscript +] cells into functional β cells.
MIT Department
Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1073/pnas.1400709111
