One-step optogenetics with multifunctional flexible polymer fibers
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Anikeeva_One-step optogenetics.pdf
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Author(s)
Jia, Xiaoting
Grena, Benjamin
Park, Seongjun
Guo, Yuanyuan
Choe, Han Kyoung
Grena, Benjamin Jean-Baptiste
Kang, Jeewoo
Park, Jiyeon
Lu, Chi
Canales, Andres
Date Issued
February 2017
Journal
Nature Neuroscience
Publisher
Nature Publishing Group
Citation
Park, Seongjun et al. “One-Step Optogenetics with Multifunctional Flexible Polymer Fibers.” Nature Neuroscience 20, no. 4 (February 20, 2017): 612–619. © 2017 Nature America, Inc part of Springer Nature
Version
Author's final manuscript
Abstract
Optogenetic interrogation of neural pathways relies on delivery of light-sensitive opsins into tissue and subsequent optical illumination and electrical recording from the regions of interest. Despite the recent development of multifunctional neural probes, integration of these modalities in a single biocompatible platform remains a challenge. We developed a device composed of an optical waveguide, six electrodes and two microfluidic channels produced via fiber drawing. Our probes facilitated injections of viral vectors carrying opsin genes while providing collocated neural recording and optical stimulation. The miniature ( < 200 μm) footprint and modest weight ( < 0.5 g) of these probes allowed for multiple implantations into the mouse brain, which enabled opto-electrophysiological investigation of projections from the basolateral amygdala to the medial prefrontal cortex and ventral hippocampus during behavioral experiments. Fabricated solely from polymers and polymer composites, these flexible probes minimized tissue response to achieve chronic multimodal interrogation of brain circuits with high fidelity.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Department of Materials Science and Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Research Laboratory of Electronics
McGovern Institute for Brain Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/NN.4510
