Structure of duplex DNA containing the cisplatin 1,2-{Pt(NH3)2}[superscript 2]+-d(GpG) crosslink at 1.77 [Angstrom] resolution
Name
Todd and Lippard J Inorg Biochem.pdf
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Author(s)
Todd, Ryan C.
Lippard, Stephen J.
Alternative Title
Structure of duplex DNA containing the cisplatin 1,2-{Pt(NH3)2}2+-d(GpG) crosslink at 1.77 Å resolution
Date Issued
April 2010
Journal
Journal of Inorganic Biochemistry
Publisher
Elsevier B.V.
Citation
Todd, Ryan C., and Stephen J. Lippard. “Structure of Duplex DNA Containing the Cisplatin 1,2-{Pt(NH3)2}[superscript 2+]-d(GpG) Cross-link at 1.77[Ångstrom] Resolution.” Journal of Inorganic Biochemistry 104.9 (2010) : 902-908.
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Author's final manuscript
Abstract
We report the 1.77-Å resolution X-ray crystal structure of a dodecamer DNA duplex with the sequence 5′-CCTCTGGTCTCC-3′ that has been modified to contain a single engineered 1,2-cis-{Pt(NH3)2}2+-d(GpG) cross-link, the major DNA adduct of cisplatin. These data represent a significant improvement in resolution over the previously published 2.6-Å structure. The ammine ligands in this structure are clearly resolved, leading to improved visualization of the cross-link geometry with respect to both the platinum center and to the nucleobases, which adopt a higher energy conformation. Also better resolved are the deoxyribose sugar puckers, which allow us to re-examine the global structure of platinum-modified DNA. Another new feature of this model is the location of four octahedral [Mg(H2O)6]2+ ions associated with bases in the DNA major groove and the identification of 124 ordered water molecules that participate in hydrogen-bonding interactions with either the nucleic acid or the diammineplatinum(II) moiety.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
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DOI of Published Version
https://doi.org/10.1016/j.jinorgbio.2010.04.005
