Mediator and cohesin connect gene expression and chromatin architecture
Name
Young_Mediator and.pdf
Size
1.98 MB
Format
Adobe PDF
Checksum (MD5)
b2f352b1368cfbeed4d7c46b608f19db
Author(s)
Kagey, Michael H.
Newman, Jamie Jennifer
Bilodeau, Steve
Zhan, Ye
Orlando, David A.
van Berkum, Nynke L.
Ebmeier, Christopher C.
Goossens, Jesse
Rahl, Peter B.
Levine, Stuart S.
Date Issued
August 2010
Journal
Nature
Publisher
Nature Publishing Group
Citation
Kagey, Michael H. et al. “Mediator and Cohesin Connect Gene Expression and Chromatin Architecture.” Nature 467.7314 (2010): 430–435.
Version
Author's final manuscript
Abstract
Transcription factors control cell-specific gene expression programs through interactions with diverse coactivators and the transcription apparatus. Gene activation may involve DNA loop formation between enhancer-bound transcription factors and the transcription apparatus at the core promoter, but this process is not well understood. Here we report that mediator and cohesin physically and functionally connect the enhancers and core promoters of active genes in murine embryonic stem cells. Mediator, a transcriptional coactivator, forms a complex with cohesin, which can form rings that connect two DNA segments. The cohesin-loading factor Nipbl is associated with mediator–cohesin complexes, providing a means to load cohesin at promoters. DNA looping is observed between the enhancers and promoters occupied by mediator and cohesin. Mediator and cohesin co-occupy different promoters in different cells, thus generating cell-type-specific DNA loops linked to the gene expression program of each cell.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/nature09380
