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Bench-Top Fabrication of Single-Molecule Nanoarrays by DNA Origami Placement
Name
acsnano.1c01150.pdf
Description
Published version
Size
9.37 MB
Format
Adobe PDF
Checksum (MD5)
adfe385e98cb07afcb723ea1b27f90ce
Author(s)
Shetty, Rishabh M
Brady, Sarah R
Rothemund, Paul WK
Hariadi, Rizal F
Gopinath, Ashwin
Date Issued
2021
Journal
ACS Nano
Publisher
American Chemical Society (ACS)
Citation
Shetty, Rishabh M, Brady, Sarah R, Rothemund, Paul WK, Hariadi, Rizal F and Gopinath, Ashwin. 2021. "Bench-Top Fabrication of Single-Molecule Nanoarrays by DNA Origami Placement." ACS Nano, 15 (7).
Version
Final published version
Abstract
Large-scale nanoarrays of single biomolecules enable high-throughput assays while unmasking the underlying heterogeneity within ensemble populations. Until recently, creating such grids which combine the advantages of microarrays and single-molecule experiments (SMEs) has been particularly challenging due to the mismatch between the size of these molecules and the resolution of top-down fabrication techniques. DNA origami placement (DOP) combines two powerful techniques to address this issue: (i) DNA origami, which provides a ∼100 nm self-assembled template for single-molecule organization with 5 nm resolution and (ii) top-down lithography, which patterns these DNA nanostructures, transforming them into functional nanodevices via large-scale integration with arbitrary substrates. Presently, this technique relies on state-of-the-art infrastructure and highly trained personnel, making it prohibitively expensive for researchers. Here, we introduce a cleanroom-free, $1 benchtop technique to create meso-to-macro-scale DNA origami nanoarrays using self-assembled colloidal nanoparticles, thereby circumventing the need for top-down fabrication. We report a maximum yield of 74%, 2-fold higher than the statistical limit of 37% imposed on non-specific molecular loading alternatives. Furthermore, we provide a proof-of-principle for the ability of this nanoarray platform to transform traditionally low-throughput, stochastic, single-molecule assays into high-throughput, deterministic ones, without compromising data quality. Our approach has the potential to democratize single-molecule nanoarrays and demonstrates their utility as a tool for biophysical assays and diagnostics.
Terms of Use
Creative Commons Attribution 4.0 International license
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DOI of Published Version
10.1021/ACSNANO.1C01150
