Production of d-Glyceric acid from d-Galacturonate in Escherichia coli
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10295_2020_2323_ReferencePDF.pdf
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Author(s)
Fox, Kevin J.
Prather, Kristala L
Date Issued
October 2020
Journal
Journal of Industrial Microbiology and Biotechnology
Publisher
Oxford University Press (OUP)
Citation
Fox, Kevin J. and Kristala L. J. Prather et al. "Production of d-Glyceric acid from d-Galacturonate in Escherichia coli." Journal of Industrial Microbiology and Biotechnology 47, 12 (October 2020): 1075–1081 © 2020 Society for Industrial Microbiology and Biotechnology
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Author's final manuscript
Abstract
A microbial production platform has been developed in Escherichia coli to synthesize d-glyceric acid from d-galacturonate. The expression of uronate dehydrogenase (udh) from Pseudomonas syringae and galactarolactone isomerase (gli) from Agrobacterium fabrum, along with the inactivation of garK, encoding for glycerate kinase, enables d-glyceric acid accumulation by utilizing the endogenous expression of galactarate dehydratase (garD), 5-keto-4-deoxy-D-glucarate aldolase (garL), and 2-hydroxy-3-oxopropionate reductase (garR). Optimization of carbon flux through the elimination of competing metabolic pathways led to the development of a ΔgarKΔhyiΔglxKΔuxaC mutant strain that produced 4.8 g/l of d-glyceric acid from d-galacturonate, with an 83% molar yield. Cultivation in a minimal medium produced similar yields and demonstrated that galactose or glycerol serve as possible carbon co-feeds for industrial production. This novel platform represents an alternative for the production of d-glyceric acid, an industrially relevant chemical, that addresses current challenges in using acetic acid bacteria for its synthesis: increasing yield, enantio-purity and biological stability.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
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DOI of Published Version
https://doi.org/10.1007/s10295-020-02323-2
