Improved precision and accuracy for microarrays using updated probe set definitions
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1471-2105-8-48.pdf
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285.36 KB
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Adobe PDF
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0deb743c8527acf4f437e9444a3601ee
Author(s)
Sandberg, Rickard
Larsson, Ola
Date Issued
February 2007
Journal
BMC Bioinformatics
Publisher
BioMed Central Ltd
Citation
Sandberg, Rickard, and Ola Larsson. “Improved precision and accuracy for microarrays using updated probe set definitions.” BMC Bioinformatics 8.1 (2007): 48.
Version
Final published version
Abstract
Background: Microarrays enable high throughput detection of transcript expression levels. Different investigators have recently introduced updated probe set definitions to more accurately map probes to our current knowledge of genes and transcripts. Results: We demonstrate that updated probe set definitions provide both better precision and accuracy in probe set estimates compared to the original Affymetrix definitions. We show that the improved precision mainly depends on the increased number of probes that are integrated into each probe set, but we also demonstrate an improvement when the same number of probes is used. Conclusion: Updated probe set definitions does not only offer expression levels that are more accurately associated to genes and transcripts but also improvements in the estimated transcript expression levels. These results give support for the use of updated probe set definitions for analysis and meta-analysis of microarray data.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1186/1471-2105-8-48
