Early tissue patterning recreated by mouse embryonic fibroblasts in a three-dimensional environment
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Quintana-2009-Early tissue pattern.pdf
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Author(s)
Quintana, Lluis
Fernandez Muinos, Teresa
Genove, Elsa
Del Mar Olmos, Maria
Borros, Salvador
Semino, Carlos E.
Date Issued
July 2009
Journal
Tissue Engineering. Part A
Publisher
Mary Ann Liebert, Inc.
Citation
Quintana, Lluís et al. “Early Tissue Patterning Recreated by Mouse Embryonic Fibroblasts in a Three-Dimensional Environment.” Tissue Engineering Part A 15.1 (2009) : 45-54. © 2009 Mary Ann Liebert, Inc.
Version
Final published version
Abstract
Cellular self-organization studies have been mainly focused on models such as Volvox, the slime mold Dictyostelium discoideum, and animal (metazoan) embryos. Moreover, animal tissues undergoing regeneration also exhibit properties of embryonic systems such as the self-organization process that rebuilds tissue complexity and function. We speculated that the recreation in vitro of the biological, biophysical, and biomechanical conditions similar to those of a regenerative milieu could elicit the intrinsic capacity of differentiated cells to proceed to the development of a tissue-like structure. Here we show that, when primary mouse embryonic fibroblasts are cultured in a soft nanofiber scaffold, they establish a cellular network that causes an organized cell contraction, proliferation, and migration that ends in the formation of a symmetrically bilateral structure with a distinct central axis. A subset of mesodermal genes (brachyury, Sox9, Runx2) is upregulated during this morphogenetic process. The expression of brachyury was localized first at the central axis, extending then to both sides of the structure. The spontaneous formation of cartilage-like tissue mainly at the paraxial zone followed expression of Sox9 and Runx2. Because cellular self-organization is an intrinsic property of the tissues undergoing development, this model could lead to new ways to consider tissue engineering and regenerative medicine.
MIT Department
Massachusetts Institute of Technology. Center for Biomedical Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1089/ten.tea.2007.0296
