Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells
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Feld_Spectroscopic phase.PDF
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Author(s)
Park, YongKeun
Yamauchi, Toyohiko
Choi, Wonshik
Dasari, Ramachandra Rao
Feld, Michael S.
Date Issued
November 2009
Journal
Optics Letters
Publisher
Optical Society of America
Citation
YongKeun Park, Toyohiko Yamauchi, Wonshik Choi, Ramachandra Dasari, and Michael S. Feld, "Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells," Opt. Lett. 34, 3668-3670 (2009)
http://www.opticsinfobase.org/abstract.cfm?URI=ol-34-23-3668
Version
Author's final manuscript
Abstract
We report a practical method for label-free quantification of specific molecules using spectroscopic imaging of sample-induced phase shifts. Diffraction phase microscopy equipped with various wavelengths of light source is used to record wavelength-dependent phase images. We first perform dispersion measurements on pure solutions of single molecular species present in the cells, such as albumin and hemoglobin (Hb). With this prior calibration of molecular specific dispersion, we demonstrate the extraction of Hb concentration from individual human red blood cells. The end point of this study is noninvasive monitoring of physiological states of intact living cells.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Physics
Massachusetts Institute of Technology. Spectroscopy Laboratory
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DOI of Published Version
http://dx.doi.org/10.1364/OL.34.003668
