Quantifying Particle Coatings Using High-Precision Mass Measurements
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Manalis_Quantifying particle.pdf
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Author(s)
Knudsen, Scott Michael
von Muhlen, Marcio G.
Manalis, Scott R.
Date Issued
February 2012
Journal
Analytical Chemistry
Publisher
American Chemical Society
Citation
Knudsen, Scott M., Marcio G. von Muhlen, and Scott R. Manalis. “Quantifying Particle Coatings Using High-Precision Mass Measurements.” Analytical Chemistry 84, no. 3 (February 7, 2012): 1240–1242.
Version
Author's final manuscript
Abstract
We present a general method to quantify coatings on microparticle surfaces based on the additional mass. Particle buoyant mass is determined in a solution with a density that is nearly equivalent to that of the core particle, reducing the magnitude and uncertainty of the measurement. Under these conditions, added material with a different density than that of the core is a larger fraction of the total buoyant mass of the coated particle. This method can resolve a buoyant mass difference between uncoated and coated particles of 1 fg. For the protein layer on the 3 μm polystyrene spheres measured herein, this is equivalent to 1/10th of a full layer.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1021/ac300034r
