FRET-Capture: A Sensitive Method for the Detection of Dynamic Protein Interactions
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Imperiali_FRET-Capture.pdf
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1.11 MB
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Author(s)
Imperiali, Barbara
Socher, Elke Ricarda
Date Issued
December 2012
Journal
ChemBioChem
Publisher
Wiley Blackwell
Citation
Socher, Elke, and Barbara Imperiali. “FRET-Capture: A Sensitive Method for the Detection of Dynamic Protein Interactions.” ChemBioChem 14, no. 1 (January 2, 2013): 53-57.
Version
Author's final manuscript
Abstract
The FRET-Capture approach exploits a bound solvatochromic fluorophore, 4-N,N-dimethylamino-1,8-naphthalimide, as a FRET donor in both inter- and intramolecular energy transfer. A unique feature of this method is the additional level of signal selectivity as the FRET signal is only turned on when the donor is specifically bound to the protein of interest, eliminating high background and false positive signals.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1002/cbic.201200700
