Rapid casting of patterned vascular networks for perfusable engineered 3D tissues
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Bhatia_Rapid casting.pdf
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Author(s)
Miller, Jordan S.
Stevens, Kelly R.
Yang, Michael T.
Baker, Brendon M.
Nguyen, Duc-Huy T.
Cohen, Daniel M.
Toro, Esteban
Galie, Peter A.
Yu, Xiang
Chaturvedi, Ritika
Alternative Title
Rapid casting of patterned vascular networks for perfusable engineered three-dimensional tissues
Date Issued
July 2012
Journal
Nature Materials
Publisher
Nature Publishing Group
Citation
Miller, Jordan S., Kelly R. Stevens, Michael T. Yang, Brendon M. Baker, Duc-Huy T. Nguyen, Daniel M. Cohen, Esteban Toro, et al. “Rapid Casting of Patterned Vascular Networks for Perfusable Engineered Three-Dimensional Tissues.” Nature Materials 11, no. 9 (July 1, 2012): 768–774.
Version
Author's final manuscript
Abstract
In the absence of perfusable vascular networks, three-dimensional (3D) engineered tissues densely populated with cells quickly develop a necrotic core. Yet the lack of a general approach to rapidly construct such networks remains a major challenge for 3D tissue culture. Here, we printed rigid 3D filament networks of carbohydrate glass, and used them as a cytocompatible sacrificial template in engineered tissues containing living cells to generate cylindrical networks that could be lined with endothelial cells and perfused with blood under high-pressure pulsatile flow. Because this simple vascular casting approach allows independent control of network geometry, endothelialization and extravascular tissue, it is compatible with a wide variety of cell types, synthetic and natural extracellular matrices, and crosslinking strategies. We also demonstrated that the perfused vascular channels sustained the metabolic function of primary rat hepatocytes in engineered tissue constructs that otherwise exhibited suppressed function in their core.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/nmat3357
