Consumption of atmospheric hydrogen during the life cycle of soil-dwelling actinobacteria
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EnvMicro_H2_paper_meredith_WHOAS_with_supple.pdf
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Author(s)
Bosak, Tanja
Klepac-Ceraj, Vanja
Tada, Kendall R.
Hansel, Colleen M.
Ono, Shuhei
Prinn, Ronald G.
Meredith, Laura Kelsey, 1982-
Rao, Deepa,Ph.D.Massachusetts Institute of Technology.
Date Issued
June 2014
Journal
Environmental Microbiology Reports
Publisher
Wiley Blackwell
Citation
Meredith, Laura K., Deepa Rao, Tanja Bosak, Vanja Klepac-Ceraj, Kendall R. Tada, Colleen M. Hansel, Shuhei Ono, and Ronald G. Prinn. “Consumption of Atmospheric Hydrogen During the Life Cycle of Soil-Dwelling Actinobacteria.” Environmental Microbiology Reports 6, no. 3 (November 20, 2013): 226–238.
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Author's final manuscript
Abstract
Microbe-mediated soil uptake is the largest and most uncertain variable in the budget of atmospheric hydrogen (H[subscript 2]). The diversity and ecophysiological role of soil microorganisms that can consume low atmospheric abundances of H[subscript 2] with high-affinity [NiFe]-hydrogenases is unknown. We expanded the library of atmospheric H[subscript 2]-consuming strains to include four soil Harvard Forest Isolate (HFI) Streptomyces spp., Streptomyces cattleya and Rhodococcus equi by assaying for high-affinity hydrogenase (hhyL) genes and quantifying H[subscript 2] uptake rates. We find that aerial structures (hyphae and spores) are important for Streptomyces H[subscript 2] consumption; uptake was not observed in S. griseoflavus Tu4000 (deficient in aerial structures) and was reduced by physical disruption of Streptomyces sp. HFI8 aerial structures. H[subscript 2] consumption depended on the life cycle stage in developmentally distinct actinobacteria: Streptomyces sp. HFI8 (sporulating) and R. equi (non-sporulating, non-filamentous). Strain HFI8 took up H[subscript 2] only after forming aerial hyphae and sporulating, while R. equi only consumed H[subscript 2] in the late exponential and stationary phase. These observations suggest that conditions favouring H[subscript 2] uptake by actinobacteria are associated with energy and nutrient limitation. Thus, H[subscript 2] may be an important energy source for soil microorganisms inhabiting systems in which nutrients are frequently limited.
MIT Department
Massachusetts Institute of Technology. Center for Global Change Science
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Earth, Atmospheric, and Planetary Sciences
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DOI of Published Version
https://doi.org/10.1111/1758-2229.12116
