High-Resolution View of the Yeast Meiotic Program Revealed by Ribosome Profiling
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Author(s)
Regev, Aviv
Brar, Gloria A.
Yassour, Moran
Friedman, Nir
Ingolia, Nicholas T.
Weissman, Jonathan S.
Date Issued
December 2011
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Brar, G. A., M. Yassour, N. Friedman, A. Regev, N. T. Ingolia, and J. S. Weissman. “High-Resolution View of the Yeast Meiotic Program Revealed by Ribosome Profiling.” Science 335, no. 6068 (February 2, 2012): 552-557.
Version
Author's final manuscript
Abstract
Meiosis is a complex developmental process that generates haploid cells from diploid progenitors. We measured messenger RNA (mRNA) abundance and protein production through the yeast meiotic sporulation program and found strong, stage-specific expression for most genes, achieved through control of both mRNA levels and translational efficiency. Monitoring of protein production timing revealed uncharacterized recombination factors and extensive organellar remodeling. Meiotic translation is also shifted toward noncanonical sites, including short open reading frames (ORFs) on unannnotated transcripts and upstream regions of known transcripts (uORFs). Ribosome occupancy at near-cognate uORFs was associated with more efficient ORF translation; by contrast, some AUG uORFs, often exposed by regulated 5′ leader extensions, acted competitively. This work reveals pervasive translational control in meiosis and helps to illuminate the molecular basis of the broad restructuring of meiotic cells.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1126/science.1215110
