Functional imaging of hemodynamic stimulus response in the rat retina with ultrahigh-speed spectral / Fourier domain OCT
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Fujimoto_Functional imaging.pdf
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Author(s)
Choi, Woo Jhon
Baumann, Bernhard
Clermont, Allen C.
Feener, Edward P.
Boas, David A.
Fujimoto, James G.
Date Issued
March 2013
Journal
Ophthalmic Technologies XXIII
Publisher
SPIE
Citation
Choi, WooJhon, Bernhard Baumann, Allen C. Clermont, Edward P. Feener, David A. Boas, and James G. Fujimoto. “Functional Imaging of Hemodynamic Stimulus Response in the Rat Retina with Ultrahigh-Speed Spectral / Fourier Domain OCT.” Edited by Fabrice Manns, Per G. Söderberg, and Arthur Ho. Ophthalmic Technologies XXIII (March 26, 2013). (SPIE proceedings; vol. 8567)
Version
Final published version
Abstract
Measuring retinal hemodynamics in response to flicker stimulus is important for investigating pathophysiology in small animal models of diabetic retinopathy, because a reduction in the hyperemic response is thought to be one of the earliest changes in diabetic retinopathy. In this study, we investigated functional imaging of retinal hemodynamics in response to flicker stimulus in the rat retina using an ultrahigh speed spectral / Fourier domain OCT system at 840nm with an axial scan rate of 244kHz. At 244kHz the nominal axial velocity range that could be measured without phase wrapping was +/-37.7mm/s. Pulsatile total retinal arterial blood flow as a function of time was measured using an en face Doppler approach where a 200μm × 200μm area centered at the central retinal artery was repeatedly raster scanned at a volume acquisition rate of 55Hz. Three-dimensional capillary imaging was performed using speckle decorrelation which has minimal angle dependency compared to other angiography techniques based on OCT phase information. During OCT imaging, a flicker stimulus could be applied to the retina synchronously by inserting a dichroic mirror in the imaging interface. An acute transient increase in total retinal blood flow could be detected. At the capillary level, an increase in the degree of speckle decorrelation in capillary OCT angiography images could also be observed, which indicates an increase in the velocity of blood at the capillary level. This method promises to be useful for the investigation of small animal models of ocular diseases. © (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
MIT Department
Massachusetts Institute of Technology. Department of Civil and Environmental Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Research Laboratory of Electronics
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1117/12.2004096
