Megakaryocytes respond during sepsis and display innate immune cell behaviors
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fimmu-14-1083339.pdf
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3.21 MB
Format
Adobe PDF
Checksum (MD5)
757d6f06ce0a7d81ed870a4727c05bc2
Author(s)
Frydman, Galit H.
Ellett, Felix
Jorgensen, Julianne
Marand, Anika L.
Zukerberg, Lawrence
Selig, Martin K.
Tessier, Shannon N.
Wong, Keith H. K.
Olaleye, David
Vanderburg, Charles R.
Date Issued
March 2, 2023
Publisher
Frontiers Media SA
Citation
Frydman, Galit H., Ellett, Felix, Jorgensen, Julianne, Marand, Anika L., Zukerberg, Lawrence et al. 2023. "Megakaryocytes respond during sepsis and display innate immune cell behaviors." 14.
Version
Final published version
Abstract
Megakaryocytes (MKs) are precursors to platelets, the second most abundant cells in the peripheral circulation. However, while platelets are known to participate in immune responses and play significant functions during infections, the role of MKs within the immune system remains largely unexplored. Histological studies of sepsis patients identified increased nucleated CD61+ cells (MKs) in the lungs, and CD61+ staining (likely platelets within microthrombi) in the kidneys, which correlated with the development of organ dysfunction. Detailed imaging cytometry of peripheral blood from patients with sepsis found significantly higher MK counts, which we predict would likely be misclassified by automated hematology analyzers as leukocytes. Utilizing in vitro techniques, we show that both stem cell derived MKs (SC MKs) and cells from the human megakaryoblastic leukemia cell line, Meg-01, undergo chemotaxis, interact with bacteria, and are capable of releasing chromatin webs in response to various pathogenic stimuli. Together, our observations suggest that MK cells display some basic innate immune cell behaviors and may actively respond and play functional roles in the pathophysiology of sepsis.
Subjects
Immunology
Immunology and Allergy
MIT Department
Massachusetts Institute of Technology. Division of Comparative Medicine
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.3389/fimmu.2023.1083339
