A Generic and Cell-Type-Specific Wound Response Precedes Regeneration in Planarians
Name
A generic and cell.pdf
Size
3.16 MB
Format
Adobe PDF
Checksum (MD5)
9e2a02a10bdb39107e309b93be149dbc
Author(s)
Wurtzel, Omri
Poirier, Amber
Satija, Rahul
Cote, Lauren Esther
Regev, Aviv
Reddien, Peter
Date Issued
December 2015
Journal
Developmental Cell
Publisher
Elsevier B.V.
Citation
Wurtzel, Omri, Lauren E. Cote, Amber Poirier, Rahul Satija, Aviv Regev, and Peter W. Reddien. “A Generic and Cell-Type-Specific Wound Response Precedes Regeneration in Planarians.” Developmental Cell 35, no. 5 (December 2015): 632–645.
Version
Author's final manuscript
Abstract
Regeneration starts with injury. Yet how injuries affect gene expression in different cell types and how distinct injuries differ in gene expression remain unclear. We defined the transcriptomes of major cell types of planarians—flatworms that regenerate from nearly any injury—and identified 1,214 tissue-specific markers across 13 cell types. RNA sequencing on 619 single cells revealed that wound-induced genes were expressed either in nearly all cell types or specifically in one of three cell types (stem cells, muscle, or epidermis). Time course experiments following different injuries indicated that a generic wound response is activated with any injury regardless of the regenerative outcome. Only one gene, notum, was differentially expressed early between anterior- and posterior-facing wounds. Injury-specific transcriptional responses emerged 30 hr after injury, involving context-dependent patterning and stem-cell-specialization genes. The regenerative requirement of every injury is different; however, our work demonstrates that all injuries start with a common transcriptional response.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.devcel.2015.11.004
