A viral strategy for targeting and manipulating interneurons across vertebrate species
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nihms820640.pdf
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Author(s)
Chen, Qian
Liu, R
Lu, C
Feng, Guoping
Date Issued
October 2016
Journal
Nature Neuroscience
Publisher
Nature Publishing Group
Citation
Dimidschstein, Jordane et al. “A Viral Strategy for Targeting and Manipulating Interneurons Across Vertebrate Species.” Nature Neuroscience 19, 12 (October 2016): 1743–1749 © 2016 Nature America, Inc., part of Springer Nature
Version
Author's final manuscript
Abstract
A fundamental impediment to understanding the brain is the availability of inexpensive and robust methods for targeting and manipulating specific neuronal populations. The need to overcome this barrier is pressing because there are considerable anatomical, physiological, cognitive and behavioral differences between mice and higher mammalian species in which it is difficult to specifically target and manipulate genetically defined functional cell types. In particular, it is unclear the degree to which insights from mouse models can shed light on the neural mechanisms that mediate cognitive functions in higher species, including humans. Here we describe a novel recombinant adeno-associated virus that restricts gene expression to GABAergic interneurons within the telencephalon. We demonstrate that the viral expression is specific and robust, allowing for morphological visualization, activity monitoring and functional manipulation of interneurons in both mice and non-genetically tractable species, thus opening the possibility to study GABAergic function in virtually any vertebrate species.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
McGovern Institute for Brain Research at MIT
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/NN.4430
