YY1 Is a Structural Regulator of Enhancer-Promoter Loops
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Jaenisch_YY1 Is a Structural Regulator of Enhancer-Promoter Loops.pdf
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Author(s)
Sigova, Alla A.
Hannett, Nancy M.
Day, Daniel S.
Abraham, Brian J.
Cohen, Malkiel A.
Nabet, Behnam
Buckley, Dennis L.
Guo, Yang Eric
Hnisz, Denes
Bradner, James E.
Date Issued
December 2017
Journal
Cell
Publisher
Elsevier BV
Citation
Weintraub, Abraham S., Charles H. Li, Alicia V. Zamudio, Alla A. Sigova, Nancy M. Hannett, Daniel S. Day, Brian J. Abraham, et al. “YY1 Is a Structural Regulator of Enhancer-Promoter Loops.” Cell 171, no. 7 (December 2017): 1573–1588.e28. © 2017 Elsevier Inc.
Version
Author's final manuscript
Abstract
There is considerable evidence that chromosome structure plays important roles in gene control, but we have limited understanding of the proteins that contribute to structural interactions between gene promoters and their enhancer elements. Large DNA loops that encompass genes and their regulatory elements depend on CTCF-CTCF interactions, but most enhancer-promoter interactions do not employ this structural protein. Here, we show that the ubiquitously expressed transcription factor Yin Yang 1 (YY1) contributes to enhancer-promoter structural interactions in a manner analogous to DNA interactions mediated by CTCF. YY1 binds to active enhancers and promoter-proximal elements and forms dimers that facilitate the interaction of these DNA elements. Deletion of YY1 binding sites or depletion of YY1 protein disrupts enhancer-promoter looping and gene expression. We propose that YY1-mediated enhancer-promoter interactions are a general feature of mammalian gene control. YY1 is a structural regulator of enhancer-promoter interactions and facilitates gene expression.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Creative Commons Attribution-NonCommercial-NoDerivs License
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DOI of Published Version
https://doi.org/10.1016/J.CELL.2017.11.008
