A CRISPR-based assay for the detection of opportunistic infections post-transplantation and for the monitoring of transplant rejection
Name
200622_nBME_CrisprDx.pdf
Description
Accepted version
Size
9.56 MB
Format
Adobe PDF
Checksum (MD5)
f1ea5d5e872174a5cd5a5e12cbd12845
Author(s)
Kaminski, Michael M.
Alcantar, Miguel A.
Lape, Isadora T.
Greensmith, Robert
Huske, Allison C.
Valeri, Jacqueline A.
Marty, Francisco M.
Klämbt, Verena
Azzi, Jamil
Akalin, Enver
Date Issued
April 2020
Journal
Nature Biomedical Engineering
Publisher
Springer Science and Business Media LLC
Citation
Kaminski, Michael M. et al. "A CRISPR-based assay for the detection of opportunistic infections post-transplantation and for the monitoring of transplant rejection." Nature Biomedical Engineering 4, 6 (April 2020): 601–609 © 2020 The Author(s)
Version
Author's final manuscript
Abstract
In organ transplantation, infection and rejection are major causes of graft loss. They are linked by the net state of immunosuppression. To diagnose and treat these conditions earlier, and to improve long-term patient outcomes, refined strategies for the monitoring of patients after graft transplantation are needed. Here, we show that a fast and inexpensive assay based on CRISPR–Cas13 accurately detects BK polyomavirus DNA and cytomegalovirus DNA from patient-derived blood and urine samples, as well as CXCL9 messenger RNA (a marker of graft rejection) at elevated levels in urine samples from patients experiencing acute kidney transplant rejection. The assay, which we adapted for lateral-flow readout, enables—via simple visualization—the post-transplantation monitoring of common opportunistic viral infections and of graft rejection, and should facilitate point-of-care post-transplantation monitoring.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Broad Institute of MIT and Harvard
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/s41551-020-0546-5
